冷泉渗漏区海底微生物作用及生物标志化合物

管红香; 陈多福; 宋之光

冷泉渗漏区海底微生物作用及生物标志化合物

1 中国科学院广州地球化学研究所边缘海地质重点实验室, 广州 510640;
2 中国科学院研究生院, 北京 100049;
3 中国科学院广州地球化学研究所有机地球化学国家重点实验室, 广州 510640

基金项目:

中国科学院知识创新工程重要方向项目(KZCX3-SW-224)

国家自然科学基金项目(40472059)

广东省自然科学基金项目(05200113)

中国科学院知识创新工程前沿领域项目(GIGCX-04-03)

详细信息

作者简介:
管红香(1981-),女,硕士生,主要从事冷泉沉积中的生物标志化合物研究,E-mail:guanhongxiang@gig.ac.cn

中图分类号: P736.2
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出版历程
- 收稿日期: 2007-03-08
- 修回日期: 2007-08-19

BIOMARKERS AND BACTERIAL PROCESSES IN THE SEDIMENTS OF GAS SEEP SITE

1 Key Laboratory of Marginal Sea Geology, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;
2 Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;
3 State Key Laboratory of Organic Geochemistry, Guangzhou 510640, China

摘要

摘要: 在有冷泉活动和水合物产出的海底环境中,甲烷氧化古细菌和硫酸盐还原细菌十分发育,它们主导着海底天然气(主要是甲烷)的缺氧氧化作用,并在海底碳循环和生物种群繁衍中发挥着重要作用。海底天然气渗漏活动区的甲烷氧化古细菌使渗漏CH₄缺氧氧化为HCO₃^-,硫酸盐还原细菌使SO₄^2-转化为HS^-,从而使细菌微生物获得生命所需的能量,生物种群得以发育和繁衍。甲烷氧化古细菌有ANME-1、ANME-2、ANME-3三个种群,形成相应的醚类异戊二烯类和类异戊二烯烃类生物标志物。硫酸盐还原细菌有Desulfosarcina和Desulfococcus两个主要的细菌群落,形成二烃基甘油二醚和脂肪酸生物标志化合物。这种天然气渗漏区内微生物活动产生的生物标志化合物都具有特别负的碳同位素组成,δ¹³C值为-41.1‰~-95.6‰,说明微生物群落在生命代谢过程中摄取了来自甲烷的碳,同时也反映了天然气渗漏系统缺氧带存在的古细菌和硫酸盐还原细菌活动。
- 生物标志化合物 /
- 冷泉 /
- 甲烷氧化古细菌 /
- 硫酸盐还原细菌
Abstract: Methane oxidizing archea (MOA) and sulfate reducing bacteria (SRB) are abundant in cold seep and hydrate sites, where dominant anaerobic oxidation of methane played an important role in the sea carbon cycle and microbial propagation. MOA oxidizes methane into HCO₃^- and SRB reduces SO₄^2- into HS^- at gas seep site, which is anaerobic, and microbes obtain energy here for living and growth. At least MOA consists of three colonies:ANME-1, ANME-2 and ANME-3, showed in biomarkers as isoprenoids and free isoprenoid hydrocarbons. There are two colonies of SRB,that is, Desulfosarcina and Desulfococcus. The typical biomarkers produced by SRB are Dialkyl glycerol diethers (DGDs) and fatty acids. All the biomarkers of cold seep sites have very low carbon isotopic compositions which are between -41.1‰~-95.6‰, indicating that the microbes get carbon from CH₄ and that there are activities of MOA and SRB in anaerobic gas seep sites.
- biomarker /
- cold-seep /
- methane oxidizing archea /
- sulfate reducing bacteria

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参考文献(45)

[1]	Pancost R D, Chopmans E, Sinninghe J S. Archaeal lipids in Mediterranean Cold Seeps:Molecular proxies for anaerobic methane oxidation[J]. Geochimica et Cosmochimica Acta, 2001, 65:1611-1627.
[2]	王家生, Suess E. 天然气水合物伴生的沉积物碳、氧同位素示踪[J].科学通报, 2002,47:1172-1176.[WANG Jia-sheng, Suess E. Indicators of δ^{13 C and δ¹⁸O of gas hydrate-associated sediments[J]. Chinese Science Bulletin, 2002,47(19):1659-1663.]}
[3]	Hinrichs K U, Hayes J M, Sylva S P, et al. Methane-consuming archaebacteria in marine sediments[J]. Nature, 1999, 398:802-805.
[4]	Boetius A, Ravenschlag K, Schubert C J, et al. A marine microbial consortium apparently mediating anaerobic oxidation of methane[J]. Nature, 2000, 407:623-626.
[5]	陈多福, 陈先沛, 陈光谦. 冷泉流体沉积碳酸盐岩的地质地球化学特征[J]. 沉积学报, 2002, 20(1):35-40. [CHEN Duo-fu, CHEN Xian-pei, CHEN Guang-qian. Geology and geochemistry of cold seepage and venting related carbonates[J]. Acta Sedimentologica Sinica, 2002, 20(1):35-40.]
[6]	Zhang C L, Li Y, Wall J D, et al. Lipid and carbon isotopic evidence of methane oxidizing and sulfate-reducing bacteria in association with gas hydrates from the Gulf of Mexico[J]. Geology, 2002, 30:239-242.
[7]	Zhang C L, Pancost R D, Sassenc R, et al. Archaeal lipid biomarkers and isotopic evidence of anaerobic methane oxidation associated with gas hydrates in the Gulf of Mexico[J]. Organic Geochemistry, 2003, 34:827-836.
[8]	Schouten S, Wakeham S G, Sinninghe Damst J S. Evidence for anaerobic methane oxidation by archaea in euxinic waters of the Black Sea[J]. Organic Geochemistry,2001,32:1277-1281.
[9]	Elvert M, Suess E, Whiticar M J. Anaerobic methane oxidation associated with marine gas hydrates:superlight C-isotopes from saturated and unsaturated C₂₀ and C₂₅ irre-gular isoprenoids[J]. Naturwissenschaften, 1999, 86:295-300.
[10]	Hinrichs K U, Sylva S P, Summons R E, et al. Molecular and isotopic analysis of anaerobic methane oxidizing communities in marine sediments[J]. Organic Geochemistry, 2000, 31:1685-1701.
[11]	Peckmann J, Thiel V. Carbon cycling at ancient methane-seeps[J]. Chemical Geology, 2004, 205:443-467.
[12]	Barber C J, Grice K, Baston T P, et al. The identification of crocetane in Australian crude oils[J]. Organic Geochemistry, 2001, 32:943-947.
[13]	Nauhaus K, Treude T, Boetius A, et al. Environmental regulation of the anaerobic oxidation of methane:a comparison of ANME-I and ANME-Ⅱ communities[J]. Environmental Microbiology, 2005, 7(1):98-106.
[14]	Hallam S J, Putnam N, Preston C M, et al. Reverse methanogenesis:Testing the hypothesis with environmental genomics[J]. Science, 2004, 305:1457-1462.
[15]	Hallam S J, Girguis P R, Preston C M, et al. Identification of methyl coenzyme M reductase A (mcrA) genes associated with methaneoxidizing Archaea[J]. Appl. Environ. Microbiol., 2003, 69:5483-5491.
[16]	冯东, 陈多福,苏正,等. 海底甲烷缺氧氧化与冷泉碳酸盐岩沉淀动力学[J]. 海洋地质与第四纪地质, 2006, 26(3):125-131. [FENG Dong, CHEN Duo-fu, SU Zheng,el al. Anaerobic oxidization of methane and seep carbonate precipitation kinetics at seafloor[J]. Marine Geology and Quaternary Geology, 2006, 26(3):125-131.]
[17]	Orphan V J, House C H, Hinrichs K U,et al. Methane-consuming Archaea revealed by directly coupled isotopic and phylogenetic analysis[J]. Science, 2001, 293:484-487.
[18]	Orcutt B, Boetius A, Elvert M, et al. Molecular biogeochemistry of sulfate reduction, methanogenesis and the anaerobic oxidation of methane at Gulf of Mexico cold seeps[J]. Geochimica et Cosmochimica Acta, 2005, 69(17):4267-4281.
[19]	Knittel K, sekann T L, Boetius A, et al. Diversity and distribution of methanotrophic Archaea at cold seeps[J]. Applied and Environmental Microbiology, 2005:467-479.
[20]	Orphan V J, Hinrichs K U, Ussler Ⅲ W, et al. Comparative analysis of methane-oxidizing Archaea and sulfate-reducing bacteria in anoxic marine sediments[J].Appl. Environ. Microbiol., 2001, 67:1922-1934.
[21]	Girguis P R, Orphan V J, Hallam S J, et al. Growth and methane oxidation rates of anaerobic methanotrophic Archaea in a continuous-flow bioreactor[J]. Appl. Environ. Microbiol., 2003, 69:5472-5482.
[22]	Lanoil B D, Sassen R, La Duc M T, et al. Bacteria and Archaea physically associated with Gulf of Mexico gas hydrates[J].Appl. Environ. Microbiol., 2001, 67:5143-5153.
[23]	Mills H J,Hodges C,Wilson K, et al. Microbial diversity in sediments associated with surface-breaching gas hydrate mounds in the Gulf of Mexico[J]. FEMS Microbiol. Ecol., 2003,46(1):39-52.
[24]	Boetius A, Ravenschlag K, Schubert C, et al. A marine microbial consortium apparently mediating anaerobic oxidation of methane[J].Nature, 2000, 407:623-626.
[25]	Elvert M, Greinert J, Seuss E, et al. Carbon isotopes of biomarkers derived from methane-oxidizing microbes at Hydrate Ridge, Cascadia Covergent Margin, in Natural Gas Hydrates:Occurance, Distribution, and Detection[C]//Geophysical Monograph Series. American Geophysical Union, 2001, 124:115-129.
[26]	Sahling H, Rickert D, Lee R W, et al. Macrofaunal community structure and sulfide flux at gas hydrate deposits from the Cascadia convergent margin, NE Pacific[J]. Mar. Ecol. Prog. Ser., 2002,231:121-138.
[27]	Colbert M D, Tryon K M, Brown, et al. Fluid and chemical fluxes in and out of sediments hosting methane hydrate deposits on Hydrate Ridge, OR. I. Hydrological provinces[J]. Earth Planet. Sci. Lett., 2002, 201:525-540.
[28]	Tryon M D, Brown K M,Torres M E. Fluid and chemical fluxes in and out of sediments hosting methane hydrate deposits on Hydrate Ridge[J]. Earth Planet. Sci. Lett., 2002, 201:541-557.
[29]	Baumgartner L K, Reid R P, Dupraz C, et al. Sulfate reducing bacteria in microbial mats:Changing paradigms, new discoveries[J]. Sedimentary Geology, 2006,185:131-145.
[30]	Orphan V J, House C H, Hinrichs K U, et al.Methane-consuming Archaea revealed by directly coupled isotopic and phylogenetic analysis[J]. Science, 2001,293:484-487.
[31]	Orphan V J, House C H, Hinrichs K U, et al. Multiple Archaeal groups mediate methane oxidation in anoxic cold seep sediments[C]//Proceedings of the National Academy of Sciences of the United States of America. 2002, 99:7663-7668.
[32]	Blumenberg M, Seifert R, Reitner J, et al. Membrane lipid patterns typify distinct anaerobic methanotrophic consortia[J]. PNAS, 2004, 30:11111-11116.
[33]	Reitner J, Peckmann J, Blumenberg M, et al. Concretionary methane-seep carbonates and associated microbial communities in Black Sea sediments[J]. Palaeogeography, Palaeoclimatology, Palaeoecology, 2005, 227:18-30.
[34]	Joye S B, Boetius A, Orcutt B N, et al. The anaerobic oxidation of methane and sulfate reduction in sediments from Gulf of Mexico cold seeps[J]. Chem. Geol., 2003, 205:219-238.
[35]	Kvenvolden K A. Gas hydrates geological perspectives and global change[J]. Geophys Rev., 1993, 31:173-187.
[36]	Thiel V, Peckmann J, Richnow H H, et al. Molecular signals for anaerobic methane oxidation in Black Sea seep carbonates and a microbial mat[J]. Marine Chemistry, 2001, 73:97-112.
[37]	Pape T, Blumenberg M, Seifert R, et al. Lipid geochemistry of methane-seep-related Black Sea carbonates[J]. Palaeogeography, Palaeoclimatology, Palaeoecology, 2005, 227:31-47.
[38]	Birgel D, Thiel V, Hinrichs K U, et al. Lipid biomarker patterns of methane-seep microbialites from the Mesozoic convergent margin of California[J]. Organic Geochemistry, 2006,37(10):1289-1302.
[39]	Bouloubassi I, Aloisi G, Pancost R D, et al. Archaeal and bacterial lipids in authigenic carbonate crusts from eastern Mediterranean mud volcanoes[J]. Organic Geochemistry, 2006, 37:484-500.
[40]	Hopmans E C, Schouten S, Pancost R D, et al. Analysis of intact tetraether lipids in archaeal cell material and sediments by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry[J]. Rapid Communications in Mass Spectrometry, 2000, 14:585-589.
[41]	冯东,陈多福,苏正,等. 海底天然气渗漏系统微生物作用及冷泉碳酸盐岩的特征[J]. 现代地质, 2005, 19(1):26-32. [FENG Dong, CHEN Duo-fu, SU Zheng, et al. Characteristics of cold seep carbonates and microbial processes in gas seep system[J]. Geoscience. 2005, 19(1):26-32.]
[42]	Bart E, Dongen V, Helen M T, et al. Well preserved Palaeogene and Cretaceous biomarkers from the Kilwa area, Tanzania[J]. Organic Geochemistry, 2006,37(5):539-557.
[43]	Stuart G,Wakeham Ellen C, Hopmans, et al. Archaeal lipids and anaerobic oxidation of methane in euxinic water columns:a comparative study of the Black Sea and Cariaco Basin[J]. Chemical Geology, 2004, 205:427-442.
[44]	Pape T, Blumenberg M, Seifert R, et al. Lipid geochemistry of methane-seep-related Black Sea carbonates[J]. Palaeogeography, Palaeoclimatology, Palaeoecology, 2005, 227:31-47.
[45]	Werne J P, Sinninghe Damste J S. Mixed sources contribute to the molecular isotopic signature of methane-rich mud breccia sediments of Kazan mudvolcano (eastern Mediterranean)[J].Organic Geochemistry, 2005, 36:13-27.