Abstract: X-ray fluorescence (XRF) core scanning on marine sediments has been widely utilized in reconstructing changes in ancient ocean and climate. However, the current XRF core scanning conducted by the International Ocean Discovery Program (IODP) only provides elemental counts, which does not meet the requirements for elemental concentration in paleoceanography. This study takes the XRF core scanning data from Site U1546 of IODP Expedition 385 in the Guaymas Basin as an example. By combining the XRF measured elemental contents of discrete sediment samples and a reference material, the relationships between concentrations and counts of various elements were obtained, allowing the conversion of elemental counts from raw XRF scanning data into concentrations. Based on the previously established age model for Site U1546, various proxies such as Ti/Al, K/Al, K/Ti, Ti/Ca, Fe/Ca, Sr/Ca, and Zn/Al were utilized to reconstruct the continental weathering intensity, terrigenous input, oceanic nutrient conditions, and primary productivity in the Guaymas Basin since the middle Pleistocene (since 350 ka). Results indicate that the continental weathering intensity shows a generally periodic change, being weaker during glacial periods and stronger during interglacial periods. Terrigenous input, nutrient availability and primary productivity fluctuated significantly between 350 and 130 ka and increased slowly since 130 ka. Nutrient availability and primary productivity were positively correlated with terrigenous input, suggesting a possible terrestrial control. This study revealed the changing patterns of continental surface weathering intensity, terrigenous material input, and marine nutrition and primary productivity in the Guaymas Basin since the Middle Pleistocene, providing an important scientific basis for further exploring the response of ancient marine environmental evolution to climate change.